Phytochemical composition of Lagenaria siceraria fruits from KwaZulu-Natal and Limpopo, South Africa.

Lagenaria siceraria (Molina) Standley is a food and medicinal source with anti-proliferative, anti-fertility, anti-HIV and anti-cancerous properties. The current study investigated the phytochemical constituents of L. siceraria fruits using gas chromatography/mass spectrometry (GC-MS). Five isoprenoids present in all investigated landraces were 1-Dodecene, 2,3-Dimethyldodecane, E-15-Heptadecenal, Eicosane, and Tridecane, 6-propyl. Lighter metabolites such as 1-Dodecene and 2,3-Dimethyldodecane were recorded at a shorter retention time range of 9.08-16.29 min over a lower relative peak area ranging from 1.09 to 6.97%. However, heavier compounds (E-15-Heptadecenal, Eicosane and Tridecane, 6-propyl) had a longer retention time range of 13.42-18.00 mins over a higher relative peak area range of 2.25-11.41%. Cluster analysis grouped landraces into 5 clusters (I -V) according to their fruit and seed attributes, and isoprenoid units significant to each cluster. Terpenoids were the prominent phytochemicals present in fruits. This is the most comprehensive study on the fruit phytochemical constituents of different L. siceraria landraces to date.

Genetic diversity and population structure of maize inbred lines using phenotypic traits and single nucleotide polymorphism (SNP) markers.

Understanding germplasm's genetic diversity is essential for developing new and improved cultivars with stable yields under diverse environments. The objective of this study was to determine the genetic diversity and population structure of 128 maize inbred lines sourced from the International Institute of Tropical Agriculture (IITA), the International Maize and Wheat Improvement Centre (CIMMYT), and the University of KwaZulu-Natal (UKZN) using 11,450 informative single nucleotide polymorphism (SNP) markers. The inbred lines revealed highly significant (p < 0.001) levels of variability for the key phenotypic traits. The SNP markers had a mean gene diversity (GD) and polymorphic information content (PIC) of 0.40 and 0.31, respectively, indicating the existence of substantial genetic variation across the germplasm panel. The model-based population structure analysis identified three subpopulations (K = 3) among the inbred lines. This corroborated the phylogenetic analysis using phenotypic traits and molecular markers which classified the inbred lines into three groups. The findings of this study identified considerable genetic diversity for the selection of inbred lines with favourable alleles for multiple traits and could be useful to initiate marker-assisted selection (MAS) to identify significant loci associated with agronomic performance and multiple-stress tolerance.

Morpho-physiological and biochemical characterization of African spider plant (Gynandropsis gynandra (L.) Briq.) genotypes under drought and non-drought conditions.

The African spider plant (Gynandropsis gynandra (L.) Briq.) is a nutrient-dense, climate-resilient indigenous vegetable with a C4 carbon fixation pathway. Understanding African spider plant drought tolerance mechanisms is essential for improving its performance in water-stressed areas. The objective of this study was to evaluate the stress tolerance potential of African spider plant accessions based on thirteen morphological, physiological, and biochemical traits under three different water treatment regimes. Eighteen accessions were evaluated over two growing seasons in the greenhouse using a split-split plot design with four replications and three water treatment-regimes namely optimum (100% field capacity), intermediate drought (50% field capacity) and, severe drought (30% field capacity). The results revealed that water regime had a significant effect (P< 0.01) on the accessions for the traits studied. A significant reduction across most of the studied traits was observed under drought conditions. However, proline content in all the accessions significantly rose under drought conditions. The principal component analysis revealed a considerable difference in the performance of the 18 African spider plant accessions under optimum and drought stress conditions. Several morphological and physiological parameters, including days to 50% flowering (r = 0.80), leaf length (r = 0.72), net photosynthesis (r = 0.76) and number of leaves per plant (r = 0.79), were positively associated with leaf yield under drought conditions. Cluster analysis categorized the 18 accessions and 13 measured parameters into 4 clusters, with cluster-1 exhibiting greater drought tolerance for most of the studied traits, and cluster-4 having the most drought-sensitive accessions. Among the accessions tested, accessions L3 and L5 demonstrated excellent drought tolerance and yield performance under both conditions. As a result, these accessions were selected as candidates for African spider plant drought tolerance breeding programs. These findings will serve as the foundation for future studies and will aid in improving food and nutrition security in the face of drought.

Agromorphological and Physiological Performance of Ethiopian Common Bean (Phaseolus vulgaris L.) Genotypes under Different Agroecological Conditions.

The objectives of this study were to assess the agronomic performance of common bean genotypes, previously selected for their response to infestation, by Mexican bean weevil and to identify promising lines that can be used as parents in a downstream breeding program. Field experiments were conducted using 144 genotypes under three different agro-ecologies in an unbalanced incomplete block design with three replications. Data on 15 agro-morphological traits were collected, and multivariate methods were used to examine the patterns of variation among the genotypes. The genotypes revealed a high level of phenotypic diversity for all agronomic traits. Six principal components, which contributed 84% of the total variation among the genotypes, were identified. The 15 agro-morphological traits classified the genotypes into three distinct major clusters and sub-clusters. The clustering patterns of the genotypes were according to the seed size, whereby the small and medium beans were distinctly separated from the large-seeded beans. The study established the existence of considerable genetic variations among common bean genotypes. Unique genotypes, such as Nasir, Awash Melka, and RAZ-36 from Cluster I, RAZ-2, RAZ-11, and RAZ-42 from Cluster II, and SER-125, SCR-15, MAZ-200, MAZ-203, and RAZ-120 from Cluster III, were selected based on their distinct agronomic performance. The selected genotypes could be useful for the common bean breeding program.

Genome-wide association mapping for component traits of drought tolerance in dry beans (Phaseolus vulgaris L.).

Understanding the genetic basis of traits of economic importance under drought stressed and well-watered conditions is important in enhancing genetic gains in dry beans (Phaseolus vulgaris L.). This research aims to: (i) identify markers associated with agronomic and physiological traits for drought tolerance and (ii) identify drought-related putative candidate genes within the mapped genomic regions. An andean and middle-american diversity panel (AMDP) comprising of 185 genotypes was screened in the field under drought stressed and well-watered conditions for two successive seasons. Agronomic and physiological traits, viz., days to 50% flowering (DFW), plant height (PH), days to physiological maturity (DPM), grain yield (GYD), 100-seed weight (SW), leaf temperature (LT), leaf chlorophyll content (LCC) and stomatal conductance (SC) were phenotyped. Principal component and association analysis were conducted using the filtered 9370 Diversity Arrays Technology sequencing (DArTseq) markers. The mean PH, GYD, SW, DPM, LCC and SC of the panel was reduced by 12.1, 29.6, 10.3, 12.6, 28.5 and 62.0%, respectively under drought stressed conditions. Population structure analysis revealed two sub-populations, which corresponded to the andean and middle-american gene pools. Markers explained 0.08-0.10, 0.22-0.23, 0.29-0.32, 0.43-0.44, 0.65-0.66 and 0.69-0.70 of the total phenotypic variability (R2) for SC, LT, PH, GYD, SW and DFW, respectively under drought stressed conditions. For well-watered conditions, R2 varied from 0.08 (LT) to 0.70 (DPM). Overall, 68 significant (p < 10-03) marker-trait associations (MTAs) and 22 putative candidate genes were identified across drought stressed and well-watered conditions. Most of the identified genes had known biological functions related to regulating the response to drought stress. The findings provide new insights into the genetic architecture of drought stress tolerance in common bean. The findings also provide potential candidate SNPs and putative genes that can be utilized in gene discovery and marker-assisted breeding for drought tolerance after validation.

Variation in Shoot, Peduncle and Fruit Growth of Lagenaria siceraria Landraces.

Lagenaria siceraria (Molina) Standley is a prominent food source as almost all its plant parts are edible. However, no studies have recorded the changes in shoots, peduncles and fruits during its growth. Hence, this study aimed to record changes in shoot traits and relate the peduncle to the fruit traits of L. siceraria landraces across different growth stages. Changes in shoots, peduncles and fruits during growth were compared within and among landraces using analysis of variance, correlation, principal component analysis, cluster analysis and heritability estimates. Almost all landraces had harvestable shoots at 42 days after sowing. Peduncles became shorter and wider as the fruits elongated. Shoots, peduncles and fruits correlated positively with each other. The informative principal components had a total variability of 84.488%, with a major contribution from shoot traits. The biplot and dendrogram clustered landraces with similar growth habits and the harvestable shoot and fruit attributes into three clusters, but KRI and NSRC formed singlets. Shoot width (60.2%) and peduncle length (55.2%) had high heritability estimates. The general low heritability estimates and genetic advances indicated the presence of non-additive gene action. This study is the first report on changes in harvested shoots and the relationship between peduncles and fruits during growth.

Genome-wide diversity analysis suggests divergence among Upper Guinea and the Dahomey Gap populations of the Sisrè berry (Syn: miracle fruit) plant (Synsepalum dulcificum [Schumach. & Thonn.] Daniell) in West Africa.

Although Synsepalum dulcificum is viewed as one of the most economically promising orphan tree crops worldwide, its genetic improvement and sustainable conservation are hindered by a lack of understanding of its evolutionary history and current population structure. Here, we report for the first time the application of genome-wide single nucleotide polymorphism genotyping to a diverse panel of S. dulcificum accessions to depict the genetic diversity and population structure of the species in the Dahomey Gap (DG) and Upper Guinea (UG) regions to infer its evolutionary history. Our findings suggest low overall genetic diversity but strong population divergence within the species. Neighbor-joining analysis detected two genetic groups in the UG and DG regions, while STRUCTURE distinguished three genetic groups, corresponding to the UG, Western DG, and Central DG regions. Application of Monmonier's algorithm revealed the existence of a barrier disrupting connectivity between the UG and DG groups. The Western DG group consistently exhibited the highest levels of nucleotide and haplotype diversities, while that of the Central DG exhibited the lowest. Analyses of Tajima's D, Fu's Fs, and Achaz Y* statistics suggest that while both UG and Central DG groups likely experienced recent expansions, the Western DG group is at equilibrium. These findings suggest a geographical structuring of genetic variation which supports the conclusion of differential evolutionary histories among West African groups of S. dulcificum. These results provide foundational insights to guide informed breeding population development and design sustainable conservation strategies for this species.

Improving drought tolerance in maize: Tools and techniques.

Drought is an important constraint to agricultural productivity worldwide and is expected to worsen with climate change. To assist farmers, especially in sub-Saharan Africa (SSA), to adapt to climate change, continuous generation of stress-tolerant and farmer-preferred crop varieties, and their adoption by farmers, is critical to curb food insecurity. Maize is the most widely grown staple crop in SSA and plays a significant role in food security. The aim of this review is to present an overview of a broad range of tools and techniques used to improve drought tolerance in maize. We also present a summary of progress in breeding for maize drought tolerance, while incorporating research findings from disciplines such as physiology, molecular biology, and systems modeling. The review is expected to complement existing knowledge about breeding maize for climate resilience. Collaborative maize drought tolerance breeding projects in SSA emphasize the value of public-private partnerships in increasing access to genomic techniques and useful transgenes. To sustain the impact of maize drought tolerance projects in SSA, there must be complementary efforts to train the next generation of plant breeders and crop scientists.

Phenotypic Variability of Root System Architecture Traits for Drought Tolerance among Accessions of Citron Watermelon (Citrullus lanatus var. citroides (L.H. Bailey).

Citron watermelon (Citrullus lanatus var. citroides) is a drought-tolerant cucurbit crop widely grown in sub-Saharan Africa in arid and semi-arid environments. The species is a C3 xerophyte used for multiple purposes, including intercropping with maize, and has a deep taproot system. The deep taproot system plays a key role in the species' adaptation to dry conditions. Understanding the root system development of this crop could be useful to identify traits for breeding water-use efficient and drought-tolerant varieties. This study compared the root system architecture of citron watermelon accessions under water-stress conditions. Nine selected and drought-tolerant citron watermelon accessions were grown under non-stress (NS) and water stress (WS) conditions using the root rhizotron procedure in a glasshouse. The following root system architecture (RSA) traits were measured: root system width (RSW), root system depth (RSD), convex hull area (CHA), total root length (TRL), root branch count (RBC), total root volume (TRV), leaf area (LA), leaf number (LN), first seminal root length (FSRL), seminal root angle (SRA), root dry mass (RDM), shoot dry mass (SDM), root−shoot mass ratio (RSM), root mass ratio (RMR), shoot mass ratio (SMR) and root tissue density (RTD). The data collected on RSA traits were subjected to an analysis of variance (ANOVA), correlation and principal component analyses. ANOVA revealed a significant (p < 0.05) accession × water stress interaction effect for studied RSA traits. Under WS, RDM exhibited significant and positive correlations with RSM (r = 0.65), RMR (r = 0.66), RSD (r = 0.66), TRL (r = 0.60), RBC (r = 0.72), FSRL (r = 0.73) and LN (r = 0.70). The principal component analysis revealed high loading scores for the following RSA traits: RSW (0.89), RSD (0.97), TRL (0.99), TRV (0.90), TRL (0.99), RMR (0.96) and RDM (0.76). In conclusion, the study has shown that the identified RSA traits could be useful in crop improvement programmes for citron watermelon genotypes with enhanced drought adaptation for improved yield performance under drought-prone environments.

Phenotypic variation in biomass and related traits among four generations advanced lines of Cleome (Gynandropsis gynandra L. (Briq.)).

Gynandropsis gynandra (spider plant) is an African traditional leafy vegetable rich in minerals, vitamins and health-promoting compounds with potential for health promotion, micronutrients supplementation and income generation for stakeholders, including pharmaceutical companies. However, information on biomass productivity is limited and consequently constrains breeders' ability to select high-yielding genotypes and end-users to make decisions on suitable cultivation and production systems. This study aimed to assess the phenotypic variability in biomass and related traits in a collection of G. gynandra advanced lines to select elite genotypes for improved cultivar development. Seventy-one advanced lines selected from accessions originating from Asia, West Africa, East Africa and Southern Africa were evaluated over two years with two replicates in a greenhouse using a 9 x 8 alpha lattice design. Significant statistical differences were observed among lines and genotype origins for all fourteen biomass and related traits. The results revealed three clusters, with each cluster dominated by lines derived from accessions from Asia (Cluster 1), West Africa (Cluster 2), and East/Southern Africa (Cluster 3). The West African and East/Southern African groups were comparable in biomass productivity and superior to the Asian group. Specifically, the West African group had a low number of long primary branches, high dry matter content and flowered early. The East/Southern African group was characterized by broad leaves, late flowering, a high number of short primary branches and medium dry matter content and was a candidate for cultivar release. The maintenance of lines' membership to their group of origin strengthens the hypothesis of geographical signature in cleome diversity and genetic driver of the observed variation. High genetic variance, broad-sense heritability and genetic gains showed the potential to improve biomass yield and related traits. Significant and positive correlations among biomass per plant, plant height, stem diameter and leaf size showed the potential of simultaneous and direct selection for farmers' desired traits. The present results provide insights into the diversity of spider plant genotypes for biomass productivity and represent key resources for further improvement in the species.

Leaf elemental composition analysis in spider plant [Gynandropsis gynandra L. (Briq.)] differentiates three nutritional groups.

Understanding the genetic variability within a plant species is paramount in implementing a successful breeding program. Spider plant (Gynandropsis gynandra) is an orphan leafy vegetable and an extraordinary source of vitamins, secondary metabolites and minerals, representing an important resource for combatting malnutrition. However, an evaluation of the leaf elemental composition, using a worldwide germplasm collection to inform breeding programs and the species valorization in human nutrition is still lacking. The present study aimed to profile the leaf elemental composition of G. gynandra and depict any potential geographical signature using a collection of 70 advanced lines derived from accessions originating from Asia and Eastern, Southern and West Africa. The collection was grown in a greenhouse using a 9 × 8 alpha lattice design with two replications in 2020 and 2021. Inductively coupled plasma-optical emission spectrometry was used to profile nine minerals contents. A significant difference (p < 0.05) was observed among the lines for all nine minerals. Microelements such as iron, zinc, copper and manganese contents ranged from 12.59-430.72, 16.98-166.58, 19.04-955.71, 5.39-25.10 mg kg-1 dry weight, respectively, while the concentrations of macroelements such as potassium, calcium, phosphorus and magnesium varied in the ranges of 9992.27-49854.23, 8252.80-33681.21, 3633.55-14216.16, 2068.03-12475.60 mg kg-1 dry weight, respectively. Significant and positive correlations were observed between iron and zinc and calcium and magnesium. Zinc, calcium, phosphorus, copper, magnesium, and manganese represented landmark elements in the genotypes. Eastern and Southern African genotypes were clustered together in group 1 with higher phosphorus, copper and zinc contents than Asian and West African lines, which clustered in group 2 and were characterized by higher calcium, magnesium and manganese contents. An additional outstanding group 3 of six genotypes was identified with high iron, zinc, magnesium, manganese and calcium contents and potential candidates for cultivar release. The genotype × year interaction variance was greater than the genotypic variance, which might translate to phenotypic plasticity in the species. Broad-sense heritability ranged from low to high and was element-specific. The present results reveal the leaf minerals diversity in spider plant and represent a baseline for implementing a minerals-based breeding program for human nutrition.

Use Patterns, Knowledge Diversity and Drivers for the Cultivation of the Miracle Plant [Synsepalum dulcificum (Schumach & Thonn.) Daniell] in Benin and Ghana.

Despite the growing interest in the miracle plant worldwide due to its numerous applications, the threats and the wild harvest of the species hamper its sustainable utilisation. Moreover, traditional knowledge so far documented on the species is limited to a narrow geographical coverage of its natural distribution range, which is West and Central Africa. This study analysed the use variation and knowledge acquisition pattern of the miracle plant among West African sociolinguistic groups and deciphered the drivers of populations' willingness and readiness to engage in cultivating the species. Semi-structured interviews were conducted with 510 respondents purposively selected from nine sociolinguistic groups in Benin and Ghana using the snowball sampling approach. Information was collected on respondents' socio-demographic profile, miracle plant ownership, plant parts used and preparation methods, knowledge of the species bioecology, perceived threats on the species, willingness to cultivate, maximum acreage to allocate to the species and maximum price to pay for a seedling. Descriptive statistics, generalized linear models, classification and regression tree models were used for data analysis. The miracle plant ownership mode depended on the age category. Sociolinguistic affiliation, level of schooling, migratory status and religion significantly affected the number of trees owned. We recorded 76 uses belonging to six use categories. The overall use-value of the miracle plant significantly varied according to the respondent sociolinguistic affiliation, main activity and religion. Men were the main source of knowledge and knowledge is mainly acquired along the family line. Knowledge related to food and social uses was mostly acquired from parents and people of the same generation, while magico-therapeutic and medicinal use-related knowledge were inherited from parents and grandparents. Sociolinguistic affiliation, awareness of taboos and market availability were the most important drivers of respondent willingness to cultivate the miracle plant. While the respondent's level of schooling and perception of plant growth rate determined the maximum acreage they were willing to allocate to the species in cultivation schemes, their main activity, sociolinguistic affiliation and knowledge of the species time to fruiting drove the maximum purchase price they were willing to offer for a seedling of the species. Our findings provide key information for the promotion of miracle plant cultivation in the study area.

SNP-based assessment of genetic purity and diversity in maize hybrid breeding.

Assessment of genetic purity of parental inbred lines and their resultant F1 hybrids is an essential quality control check in maize hybrid breeding, variety release and seed production. In this study, genetic purity, parent-offspring relationship and diversity among the inbred lines were assessed using 92 single-nucleotide polymorphism (SNP) markers. A total of 188 maize genotypes, comprising of 26 inbred lines, four doubled haploid (DH) lines and 158 single-cross maize hybrids were investigated in this study using Kompetitive Allele Specific Polymerase Chain Reaction (KASP) genotyping assays. The bi-allelic data was analyzed for genetic purity and diversity parameters using GenAlex software. The SNP markers were highly polymorphic and 90% had polymorphic information content (PIC) values of > 0.3. Pairwise genetic distances among the lines ranged from 0.05 to 0.56, indicating a high level of dissimilarity among the inbred lines. A maximum genetic distance of (0.56) was observed between inbred lines CKDHL0089 and CML443 while the lowest (0.05) was between I-42 and I-40. The majority (67%) of the inbred lines studied were genetically pure with residual heterozygosity of <5%, while only 33% had heterozygosity levels of >5%. Inbred lines, which were not pure, require purification through further inbreeding. Cluster analysis partitioned the lines into three distinct genetic clusters with the potential to contribute new beneficial alleles to the maize breeding program. Out of the 68 hybrids (43%) that passed the parent-offspring test, seven hybrids namely; SCHP29, SCHP95, SCHP94, SCHP134, SCHP44, SCHP114 and SCHP126, were selected as potential candidates for further evaluation and release due to their outstanding yield performance.

Comparative analysis of management practices and end-users' desired breeding traits in the miracle plant [Synsepalum dulcificum (Schumach & Thonn.) Daniell] across ecological zones and sociolinguistic groups in West Africa.

BACKGROUND: Understanding end-users' preferred breeding traits and plant management practices is fundamental in defining sound breeding objectives and implementing a successful plant improvement programme. Since such knowledge is lacking for Synsepalum dulcificum, a worldwide promising orphan fruit tree species, we assessed the interrelationships among socio-demography, ecology, management practices, diversity and ranking of desired breeding traits by end-users of the species (farmers, final consumers and processing companies) in West Africa. METHODS: Semi-structured interviews, field-visits and focus groups were combined to interview a total of 300 farmers and final consumers belonging to six sociolinguistic groups sampled from three ecological zones of Benin and Ghana. One processing company in Ghana was also involved. Data collected included socio-demographic characteristics; crop management systems and practices; and preferences of farmers, final consumers and processing companies and ranking of breeding traits. Data were analysed using descriptive statistics, independence, and non-parametric tests, generalized linear models, multi-group similarity index and Kendall's concordance coefficient. RESULTS: Men (86.33% of respondents) were the main holders of S. dulcificum in the study area. The three most frequent management practices observed in the species included weeding, fertilization and pruning, which were applied by 75.66%, 27.33% and 16.66% of respondents, respectively. The management intensity index varied significantly across ecological zones, sociolinguistic groups, and instruction level (p < 0.001) but was not affected by gender (p > 0.05). General multigroup similarity indices ([Formula: see text]) for farmer-desired traits, on one hand, and final consumer-desired traits, on the other hand, were high across ecological zones ([Formula: see text] ≥ 0.84) and sociolinguistic groups ([Formula: see text] > 0.83). Nevertheless, respondents from the Guineo-Congolian (Benin) and the Deciduous forest (Ghana) zones expressed higher agreement in the ranking of desired breeding traits. Preference for breeding traits was 60% similar among farmers, final consumers, and processors. The key breeding traits desired by these end-users included in descending order of importance big fruit size, early fruiting, high fruit yielding (for farmers); big fruit size, high fruit miraculin content, fruit freshness (for final consumers); and high fruit miraculin content, big fruit size, high fruit edible ratio (for processing companies). CONCLUSION: This study revealed stronger variations in current management practices across ecological zones than across sociolinguistic groups. A high similarity was shown in end-users' preferences for breeding traits across the study area. Top key traits to consider in breeding varieties of S. dulcificum to meet various end-users' expectations in West Africa include fruit size and fruit miraculin content. These results constitute a strong signal for a region-wide promotion of the resource.

Farmers' perceptions of navy bean (Phaseolus vulgaris L.) production constraints, preferred traits and farming systems and their implications on bean breeding: a case study from South East Lowveld region of Zimbabwe.

BACKGROUND: Navy bean is an important legume crop in Zimbabwe. Although its production in Zimbabwe is limited by multiple constraints including biotic, abiotic and socio-economic, there is no documented evidence. Thus, this study aimed at identifying farmers' production constraints, preferred traits and cultivars of navy bean, and strategies used to mitigate some of these constraints. METHODS: A Participatory Rural Appraisal approach involving transect walks, focus group discussions (FGDs), and formal surveys with semi-structured questionnaires was conducted in four villages of the Lowveld region of Zimbabwe. In each of the four villages, two FGDs (one for men and one for women) were conducted. A total of 176 (75 males and 101 females) navy bean-growing households were interviewed. Data from household interviews and FGDs was analysed using the Statistical Package for Social Scientists computer package. RESULTS: The most important constraints to navy bean production were drought stress (Females-86%, Males-73%), heat stress (Females-58%, Males-55%), power outages (Females-46%, Males-54%), poor soil fertility (Females-32%; Males-33%) and susceptibility to pod shattering (Females-32%, Males-43%). Mitigation strategies included mulching (18%), ridges (12%), reduced acreage (11%), and cultivating to retain more soil moisture (11%) for drought stress, while irrigating at night (32%), and adjusting planting dates (29%) were used to manage heat stress. Farmer-preferred traits included tolerance to drought and heat, early maturing varieties and disease resistance. Marketing constraints included non-payment for produce in hard currency, lack of diversity in terms of off-takers, high inflation, low grain producer price, delayed payment and breach of contract by contractors. CONCLUSION: There will be increased adoption of improved navy bean cultivars if breeding programs address the aforementioned constraints and consider farmer-preferred traits when developing new cultivars. Breeders should work closely with extension officers to ensure that cultivars released are cultivated with appropriate agronomic packages for increased productivity and high adoption.

Introgression of bruchid (Zabrotes subfasciatus) resistance into small red common bean (Phaseolus vulgaris) background and validation of the BRU_00261 (snpPV0007) resistance marker.

Bruchids are a major storage pest of common bean. Genetic resistance is a suitable method to avoid grain losses during storage. The objective of the study was to introgress the arcelin-based resistance locus into selected advanced breeding line and to validate the molecular marker BRU_00261. A total of 208 progeny F4 families were phenotyped using a randomized complete block design, with three replications. Highly significant differences (P < .001) among the entries, parents and offspring were recorded for almost all traits. There was no significant difference between the two parents in the number of eggs laid. The progenies were grouped as highly resistant (34.3%), resistant (11.9%), moderately resistant (21.4%) and susceptible (32.4%). The levels of broad sense heritability ranged from 68.5%-93.9% for all the traits. Eighty-three most resistant lines and the parental lines were genotyped with the marker BRU_00261 (snpPV0007). The marker segregation deviated significantly from the expected independent segregation towards a strong enrichment for the resistant marker in the selected families. This marker will be useful for selecting promising materials in early generations and phenotypic confirmation of positive lines in later generations.

Analysis of Genetic Diversity and Population Structure of Pigeonpea [Cajanus cajan (L.) Millsp] Accessions Using SSR Markers.

Understanding the genetic diversity present amongst crop genotypes is an efficient utilization of germplasm for genetic improvement. The present study was aimed at evaluating genetic diversity and population structure of 48 pigeonpea genotypes from four populations collected from diverse sources. The 48 pigeonpea entries were genotyped using 33 simple sequence repeat (SSR) markers that are polymorphic to assess molecular genetic diversity and genetic relatedness. The informative marker combinations revealed a total of 155 alleles at 33 loci, with an average of 4.78 alleles detected per marker with the mean polymorphic information content (PIC) value of 0.46. Population structure analysis using model based revealed that the germplasm was grouped into two subpopulations. The analysis of molecular variance (AMOVA) revealed that 53.3% of genetic variation existed within individuals. Relatively low population differentiation was recorded amongst the test populations indicated by the mean fixation index (Fst) value of 0.032. The Tanzanian pigeonpea germplasm collection was grouped into three major clusters. The clustering pattern revealed a lack of relationship between geographic origin and genetic diversity. This study provides a foundation for the selection of parental material for genetic improvement.

Phenotypic variation, functional traits repeatability and core collection inference in Synsepalum dulcificum (Schumach & Thonn.) Daniell reveals the Dahomey Gap as a centre of diversity.

The miracle plant Synsepalum dulcificum is a multipurpose natural sweetener and a promising West African orphan fruit shrub candidate for genetic improvement. Unfortunately, basic knowledge such as phenotypic variation and inheritance estimates required for implementing a breeding program are still lacking. A set of 203 accessions were sampled in two habitats from seven populations spread across the Dahomey Gap (DG) and the Upper Guinea forest (UG) in West Africa. The phenotypic diversity and allometric relationships among functional traits were analysed; the broad-sense heritability was estimated for fruit-traits, and a mini-core collection was developed in the species. Quantitative variation in tree- and fruit-traits was recorded, and multivariate analyses were performed to assess relationships among accessions, whereas heritability was estimated using the coefficient of repeatability. Tree-traits observed in S. dulcificum were more variable than fruit-traits. While habitat-type only affected tree-traits, the provenance population significantly affected both fruit- and tree-traits, with the UG populations outperforming the DG ones. Significant correlations were observed among fruit-traits on one hand, and among tree-traits on the other hand, whereas poor correlations were observed between tree- and fruit-traits. The multivariate analysis grouped accessions in three clusters. Promising individuals for high fruit mass and pulp-dense genotypes' selection were identified within clusters. Repeatability estimates for fruit-traits ranged from 0.015 (edible ratio) to 0.88 (fruit mass). The Core Hunter algorithm enabled the extraction of 41 individuals as robust representatives of the initial set of 203 accessions, and the mapping of this core collection suggested Dahomey Gap as a centre of diversity of the species. These original findings offer opportunities, not only for the genetic improvement of S. dulcificum, but also for targeted ex-situ conservation in the species.

Genetic diversity, SNP-trait associations and genomic selection accuracy in a west African collection of Kersting's groundnut [Macrotyloma geocarpum(Harms) Maréchal & Baudet].

Understanding the mechanisms governing complex traits variation is a requirement for efficient crop improvement. In this study, the molecular characterization, marker-trait associations and the possibility for genomic selection in a collection of 281 Kersting's groundnut accessions were carried out. The diversity panel was phenotyped using an Alpha lattice design with two replicates in two contrasting environments. Accessions were genotyped using genotyping by sequencing technology. Genome-wide association analyses were performed between single nucleotide polymorphism markers and yield-related traits across tested environments. SNP markers were used to calculate the observed (Ho) and expected heterozygosity (He), and the total gene diversity (Ht). Genetic differentiation among accessions across ecological regions of origin was analysed. Our results revealed 493 quality SNPs of which 113 had a minor allele frequency>0.05, a total gene diversity of 0.43 and average Ho and He values of 0.04 and 0.22, respectively. Four clusters, highly differentiated by seed coat colour (Fst = 0.79), were identified. The population structure analysis showed two subpopulations with high differentiation across ecological regions (Fst = 0.37). The GWAS revealed 10 significant marker-trait associations, of which six SNPs were consistent across environments. The genomic selection through cross-validation showed moderate to high prediction accuracies for leaflet length, seed dimension traits, 100 seed weight, days to 50% flowering and days to maturity. This demonstrates the existence of genetic variability within Kersting's groundnut and shows the potential for the improvement of the species. The findings also provide a first insight into the phenotype-to-genotype relationships in Kersting's groundnut, using SNP markers.

Screening Andean Diversity Panel Dry Bean Lines for Resistance to Bacterial Brown Spot Disease Under Field Conditions in South Africa.

Bacterial brown spot (BBS) disease caused by Pseudomonas syringae pv. syringae is an important disease of dry bean (Phaseolus vulgaris L.), with grain yield losses of 55% reported in South Africa. This study aimed to identify BBS disease-resistant genotypes from 415 Andean Diversity Panel (ADP) dry bean lines and 5 check cultivars under field conditions across three sites in South Africa: Warden and Middelburg under natural infestation and Potchefstroom under artificial inoculation. Plants at Potchefstroom were inoculated with P. syringae pv. syringae using three isolates at 21, 28, and 36 days after planting, and disease scoring was done at 7, 14, and 21 days after inoculation following a modified 1 to 9 International Centre for Tropical Agriculture (CIAT) scale. The BBS severity percentage and the area under the disease progress curve (AUDPC) were applied to quantify the reaction of bean genotypes to BBS disease. The study identified 17.2% of evaluated germplasm as resistant and 45.3% as moderately resistant. Genotypes ADP-0592, ADP-0790, ADP-0120, and ADP-0008 were selected for both resistance and high seed yield across the three environments. Genotypes ADP-0546, ADP-0630, ADP-0120, and ADP-0279 were selected for both high yield and resistance at Warden, whereas ADP-0038, ADP-0721, and ADP-0790 were selected for both traits at Middelburg, and lastly, ADP-0120 and ADP-0079 were selected for both traits at Potchefstroom. The best genotypes selected for both high yield and BBS resistance had grain yield >1.45 t ha-1 across sites and >1.85 t ha-1 at individual sites, and they out yielded the best-performing check cultivar (1.13 t ha-1) and the grand mean yield (0.87 t ha-1) across sites. The AUDPC had a strong negative correlation (r = -0.55, P < 0.001) with grain yield at Potchefstroom. Medium-seeded genotypes showed a lower AUDPC than the large-seeded genotypes, and indeterminate genotypes showed a lower AUDPC than determinate genotypes. The genotypes selected for resistance and yield can be utilized in future dry bean improvement efforts for the South African bean market.